My reaction to your title was that unless you're doing a lot of sleeping around while on the road it probably won't affect overpopulation problems ...
However, in regards to traffic / crowd problems, yes that does affect where me and my other half go - in fact all our trips (and our choice of where to live) are based around going to places where there aren't many people.
Being on a small motorbike makes a huge difference though, you can flow through traffic like water around the rocks in a stream - for me going into Barçelona by bike takes half the time of going by car.
On another note, we went to Venice once and it was fantastic because we went when it was (comparatively) quiet; we stayed and commuted in by bus with people who work there, which gives one a different point of view - so these things can be done with a little planning and thinking laterally.
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